Simulation of double-stranded branch point migration

Branch Migration Prevents DNA Loss during Double-Strand Break Repair

The repair of DNA double-strand breaks must be accurate to avoid genomic rearrangements that can lead to cell death and disease. This can be accomplished by promoting homologous recombination between correctly aligned sister chromosomes. Here, using a unique system for generating a site-specific DNA double-strand break in one copy of two replicating Escherichia coli sister chromosomes, we analy...

Sequencing of Large Double - Stranded

The dideoxy sequencing technique has been applied to the d i rec t sequencing of large double-stranded DNA molecu les with a small single-stranded primer. For ins tance , the method was applied to the lambda genome, which contains 48 502 base-pairs (Sanger F, Coulson AR, Hong GF, Hill D & Petersen GB, 1982, J. Mol. Biol., in press), and the coding region for gene W identified. The procedure pro...

The influence of phosphorothioate on charge migration in single and double stranded DNA: a theoretical approach.

In this study the influence of the phosphorothioate internucleotide bond on the electronic properties of single and double-stranded short nucleotides has been investigated at the M06-2X/6-31+G** level of theory in the gaseous phase. Due to the chirality of the phosphorus atom in a phosphorothioate (PT) internucleotide diester bond, the adiabatic/vertical mode of electron affinity/ionization pot...

Double-stranded RNA poses puzzle.

Microglia Recognize Double-Stranded RNA